AP05 Schütz: Conformational switching in viral regulatory proteins

Liver cells infected with Hepatitis B express a small regulatory protein that has been implicated in the carcinogenicity of the virus. There is still very little structural information available on this protein –aptly named X– which is thought to be intrinsically disordered. However, X may display propensity for secondary structure under certain experimental conditions. In this subproject, our goal is to identify conditions inducing conformational order in X. To probe the effect of post-translational modifications and binding partners, we combine solution-state NMR spectroscopy with small angle Xray scattering and mass spectrometry